General Cell Lines and Cell Cultures
Eurofins Discoverx Ready-to-Assay BB3 Bombesin Receptor Frozen Cells, 2 Vials of Cells. (HTS160RTA)
The Ready-to-Assay BB3 Bombesin Receptor Frozen Cells are a simple, thaw-and-assay cell format for use in studying drug candidates (small molecules or biologics) targeting GPCRs. This assay assesses ligand (e.g. bombesin) based activation of BRS3 GPCR activity via detection of increasing levels of intracellular calcium. The included cell line overexpresses BRS3 alongside endogenous high levels of a promiscuous G-protein, coupling the receptor to the calcium signaling pathway. Upon activation of BRS3, the resulting calcium activity can be fluorescently assayed. Ready-to-assay kits consist of two vials of the stably modified clonal cell line in a convenient thaw-and-use format. For detailed product information, User manual and datasheet, visit www.discoverx.com/products-applications/frozen-cells.
Eurofins Discoverx Ready-to-Assay GIP Glucagon Receptor Frozen Cells, 2 Vials of Cells. (HTS134RTA)
The Ready-to-Assay GIP Glucagon Receptor Frozen Cells are a simple, thaw-and-assay cell format for use in studying drug candidates (small molecules or biologics) targeting GPCRs. This assay assesses ligand (e.g. GIP) based activation of GIPR GPCR activity via detection of increasing levels of intracellular calcium. The included cell line overexpresses GIPR alongside exogenous levels of a promiscuous G-protein, coupling the receptor to the calcium signaling pathway. Upon activation of GIPR, the resulting calcium activity can be fluorescently assayed. Ready-to-assay kits consist of two vials of the stably modified clonal cell line in a convenient thaw-and-use format. For detailed product information, User manual and datasheet, visit www.discoverx.com/products-applications/frozen-cells.
Eurofins Discoverx Ready-to-Assay Motilin Receptor Frozen Cells, 2 Vials of Cells. (HTS121RTA)
The Ready-to-Assay Motilin Receptor Frozen Cells are a simple, thaw-and-assay cell format for use in studying drug candidates (small molecules or biologics) targeting GPCRs. This assay assesses ligand (e.g. motilin) based activation of MLNR GPCR activity via detection of increasing levels of intracellular calcium. The included cell line overexpresses MLNR alongside endogenous high levels of a promiscuous G-protein, coupling the receptor to the calcium signaling pathway. Upon activation of MLNR, the resulting calcium activity can be fluorescently assayed. Ready-to-assay kits consist of two vials of the stably modified clonal cell line in a convenient thaw-and-use format. For detailed product information, User manual and datasheet, visit www.discoverx.com/products-applications/frozen-cells.
Eurofins Discoverx Ready-to-Assay BB1 Bombesin Receptor Frozen Cells, 2 Vials of Cells. (HTS123RTA)
The Ready-to-Assay BB1 Bombesin Receptor Frozen Cells are a simple, thaw-and-assay cell format for use in studying drug candidates (small molecules or biologics) targeting GPCRs. This assay assesses ligand (e.g. bombesin, neuromedin B) based activation of NMBR GPCR activity via detection of increasing levels of intracellular calcium. The included cell line overexpresses NMBR alongside endogenous high levels of a promiscuous G-protein, coupling the receptor to the calcium signaling pathway. Upon activation of NMBR, the resulting calcium activity can be fluorescently assayed. Ready-to-assay kits consist of two vials of the stably modified clonal cell line in a convenient thaw-and-use format. For detailed product information, User manual and datasheet, visit www.discoverx.com/products-applications/frozen-cells.
Eurofins Discoverx ChemiScreen M4 Muscarinic Acetylcholine Receptor Stable Cell Line, 2 vials. (HTS117C)
The ChemiScreen M4 Muscarinic Acetylcholine Receptor Stable Cell Line is an engineered cell line for use in studying drug candidates (small molecules or biologics) targeting GPCRs. This cell line enables assessment of ligand (e.g. acetylcholine) based activation of CHRM4 GPCR activity via detection of increasing levels of intracellular calcium. The included cell line overexpresses CHRM4 alongside endogenous high levels of the promiscuous G protein, Gα15, coupling the receptor to the calcium signaling pathway. Upon activation of CHRM4, the resulting calcium activity can be fluorescently assayed. This stable modified cell line is provided as two vials of cryopreserved cells. For detailed product information, visit www.discoverx.com/targets/gpcr-target-biology.
ATCC Salmonella enterica; subspecies enterica; serovar Typhimurium; Strain: SAP17-7299
Salmonella enterica subsp. enterica serovar Typhimurium strain SAP17-7299 was isolated in the United States from human feces. This whole-genome sequenced bacterium was part of the 2017 PulseNet-GenomeTrakr joint proficiency test exercise. This strain has applications in infectious disease research and zoonotic disease research.
ATCC Primary Small Airway Epithelial Cells; Asthma, Human
Primary small airway epithelial cells are spindle-shaped that were isolated from the respiratory tract of a donor with asthma. The cells have an application in microbial (eg, viral, bacterial) infection and pathogenesis, airway inflammation and wound healing, asthma, pulmonary fibrosis, chronic obstructive pulmonary disease, chronic bronchitis, toxicology, and testing of pharmaceuticals.
ATCC Streptococcus agalactiae; Quantitative DNA
ATCC Genuine Nucleics can be used for assay development, verification, validation, monitoring of day-to-day test variation and lot-to-lot performance of molecular-based assays. The quantitative format allows for the generation of a standard curve for quantitative PCR (qPCR) to determine bacterial load and for other molecular biology applications.
ATCC Merck Pneumococcal polysaccharide powder Type 57 (d19A) 200mg
Purified pneumococcal polysaccharides are manufactured by Merck & Company for small quantity distribution by ATCC. The pneumococcal powders include those types which are components of the current 23-valent vaccine against Streptococcus pneumoniae.Each of the polysaccharide antigens used in the polyvalent vaccine is extracted and purified separately. These vaccine products can be used to assay for type-specific antibodies, research immune responses and study immunodeficiencies.
ATCC Merck Pneumococcal polysaccharide powder Type 23 (d23F) 2mg
This purified pneumococcal polysaccharides was manufactured by Merck & Company for small-quantity distribution by ATCC. The pneumococcal powders include types that are components of the current 23-valent vaccine against Streptococcus pneumoniae. Each of the polysaccharide antigens used in the polyvalent vaccine is extracted and purified separately. These vaccine products can be used to assay for type-specific antibodies, research immune responses, and study immunodeficiencies.
ATCC Borrelia burgdorferi; Quantitative DNA
Quantitative genomic DNA from Borrelia burgdorferi strain B31 can be used for assay development, verification, and validation as well as monitoring of day-to-day test variation and lot-to-lot performance of molecular-based assays. The quantitative format allows for the generation of a standard curve for quantitative PCR (qPCR) to determine bacterial load and for other molecular biology applications.
ATCC Cryptococcus gattii; A1MR272
An ampoule containing viable cells (may include spores and mycelia) suspended in cryoprotectant.
ATCC Salmonella enterica; subsp. enterica; serovar Enteritidis
Salmonella enterica subsp. enterica serovar Enteritidis strain NCTC 4444 was isolated from a case of gastroenteritis in Copenhagen. This whole-genome sequenced bacterial strain has applications in emerging infectious disease research and enteric disease research.
ATCC Clostridioides difficile
This strain of Clostridioides difficile was isolated from a clinical specimen. It is toxigenic (toxinotype XII) and was confirmed by PCR to have the tcdA and tcdB genes. This product has applications in enteric disease and infectious disease research.
Eurofins Discoverx Ready-to-Assay M2 Acetylcholine (Muscarinic) Receptor Frozen Cells, 2 Vials of Cells. (HTS115RTA)
The Ready-to-Assay M2 Acetylcholine (Muscarinic) Receptor Frozen Cells are a simple, thaw-and-assay cell format for use in studying drug candidates (small molecules or biologics) targeting GPCRs. This assay assesses ligand (e.g. acetylcholine) based activation of CHRM2 GPCR activity via detection of increasing levels of intracellular calcium. The included cell line overexpresses CHRM2 alongside endogenous high levels of a promiscuous G-protein, coupling the receptor to the calcium signaling pathway. Upon activation of CHRM2, the resulting calcium activity can be fluorescently assayed. Ready-to-assay kits consist of two vials of the stably modified clonal cell line in a convenient thaw-and-use format. For detailed product information, User manual and datasheet, visit www.discoverx.com/products-applications/frozen-cells.